Abstract The objective was to evaluate the adaptability and yield stability of different peach rootstock genotypes in subtropical climate in an experiment in Chapecó, Santa Catarina, Brazil. Twenty-one rootstocks under the scion cultivar 'BRS Libra' and one genotype from self-rooted nursery trees were evaluated. The 22 genotypes were evaluated as fruit set and fruit yield in the growing seasons 2014/15, 2015/16, 2016/17 and 2017/18. Adaptability and stability were measured by the GGE biplot method. In the four years of evaluation, plants from self-rooted nursery trees had higher fruit set, yield as well as greater stability. On the other hand, the instability of plants grown on rootstocks from species other than the scion cultivar was greater. It can be concluded that under the tested conditions, the self-rooted genotype of 'BRS Libra' is a potential alternative for peach growers.